| Catalog No. |
Size |
Price |
Figures |
A00865-40 ug
| 40 ug | $ 69.00 | MSDS: 20080228042104 (PDF)
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A00865-200 ug
| 200 ug | $ 311.00 |
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| Full Name | | THETM Anti-GST Monoclonal Antibody (Mouse) |
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| Alias | Anti-GST monoclonal antibody |
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| Description | Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest. |
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| Key Features |
GenScript THETM Anti-GST Monoclonal Antibody (Mouse) has high sensitivity, better than the antibody on the market.
GenScript THETM Anti-GST Monoclonal Antibody (Mouse) can recognize N-terminal, C-terminal and internal GST tag in fusion proteins. |
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| Ig Subclass | Mouse IgG1 |
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| Storage | THETM Anti-GST Monoclonal Antibody (Mouse) should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored 2-8°C. The reconstituted antibody can be stored for up to three months 2-8°C or for up to two years at or below -20°C. Avoid repeated freezing and thawing cycles. |
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| Specificity | THETM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins. |
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| Concentration | 40 μg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide and 100 μg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
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| Clone | A00865.01 |
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| Immunogen | Recombinant GST protein (26 kD) |
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| Purification Method | Protein A affinity column |
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| Reconstitution | Reconstitute the lyophilized antibody (40 μg or 100 μg) with 100 μl of deionized water to a final antibody concentration of 0.4 mg/ml or 1 mg/ml. |
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| Applications | The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibodyaffinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Western blot using One-Step WesternTM Kit: For quick results, GenScript One-Step WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2 ml of PBST).
Immunoprecipitation (IP): 1 µg/ml
Other applications: user-optimized |
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| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells. |
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| Conjugation | Unconjugated |
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| Host Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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