| Catalog No. |
Size |
Price |
Figures |
A00866-40 ug
| 40 ug | $ 74.00 | EXAMPLE:
![Mouse Anti-GST [HRP] Monoclonal Antibody](http://www.genscript.com/site2/document/3710_20080108024245.JPG "Mouse Anti-GST [HRP] Monoclonal Antibody")
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A00866-200 ug
| 200 ug | $ 333.00 |
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| Full Name | | THETM Anti-GST [HRP] Monoclonal Antibody (Mouse) |
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| Alias | Mouse Anti-GST [HRP] mAb |
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| Description | GenScript THETM Anti-GST [HRP] Monoclonal Antibody (Mouse) is highly immunoaffinity purified from mice ascites by protein A and then conjugated to horseradish peroxidase. Anti-GST [HRP] Monoclonal Antibody is suitable for detecting the expression level of GST in fusion proteins. |
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| Ig Subclass | Mouse IgG1
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| Storage | GenScript THETM Anti-GST [HRP] Monoclonal Antibody (Mouse) is best stored lyophilized form until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. Reconstituted GenScript Mouse Anti-GST can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript THETM Anti-GST [HRP] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
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| Concentration | 40 μg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal. |
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| Clone | A00866.01 |
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| Immunogen | Recombinant GST protein (26 kD) |
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| Purification Method | Protein A affinity column |
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| Reconstitution | Reconstitute the lyophilized GenScript THETM Anti-GST [HRP] (40 μg) with 100 μl deionized water to a final concentration of 0.4 mg/ml.
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| Applications | Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Other applications: user-optimized |
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| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
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| Conjugation | Horseradish peroxidase (HRP) |
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| Host Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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