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THE TM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse)  |
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| Catalog No. |
Size |
Price |
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A00867-40 ug
| 40 ug | $ 74.00 | MSDS: 20080228042254 (PDF)
EXAMPLE:
![Western Analysis using Mouse Anti GST Monoclonal Antibody [Biotin] (A00867)](http://www.genscript.com/site2/document/3623_20071122031419.JPG "Western Analysis using Mouse Anti GST Monoclonal Antibody [Biotin] (A00867)")
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A00867-200 ug
| 200 ug | $ 333.00 |
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| Full Name | | THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse) |
| | Alias | Mouse Anti-GST-tag [Biotin] mAb |
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| Description | GenScript THETM Anti-GST [Biotin] Monoclonal Antibody (Mouse) is the biotin conjugated THETM Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of GST in fusion proteins. |
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| Ig Subclass | IgG1, k |
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| Storage | THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored 2-8°C. The reconstituted antibody can be stored for up to three months 2-8°C or for up to two years at or below -20°C. Avoid repeated freeze and thaw cycles. |
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| Specificity | THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins. |
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| Concentration | 40 μg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide and 100 μg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
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| Clone | 2F10B9 |
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| Immunogen | Recombinant GST protein (26 kD) |
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| Purification Method | Protein A affinity column |
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| Reconstitution | Reconstitute the lyophilized antibody (40 µg, 100 µg, or 200 µg) with 80 µl, 200 µl, or 400 µl of deionized water to the final concentration of 0.5 mg/ml. |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
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| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells |
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| Conjugation | Biotin |
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| Host Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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| * For Non-Clinical Research Use Only *
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
