| Catalog No. |
Size |
Price |
Figures |
A00867-40 ug
| 40 ug | $ 74.00 | MSDS: 20080228042254 (PDF)
EXAMPLE:
![Western Analysis using Mouse Anti GST Monoclonal Antibody [Biotin] (A00867)](http://www.genscript.com/site2/document/3623_20071122031419.JPG "Western Analysis using Mouse Anti GST Monoclonal Antibody [Biotin] (A00867)")
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A00867-200 ug
| 200 ug | $ 333.00 |
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| Full Name | | THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse) |
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| Alias | Anti-GST-tag [Biotin] |
|---|
| Description | THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse) is highly immunoaffinity purified from mice ascites by protein A and then conjugated to biotin. The antibody is suitable for detecting the expression level of GST in fusion proteins. |
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| Ig Subclass | Mouse IgG1 |
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| Storage | THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored 2-8°C. The reconstituted antibody can be stored for up to three months 2-8°C or for up to two years at or below -20°C. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript THETM Anti-GST-tag [Biotin] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins. |
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| Concentration | 100 μg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
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| Clone | A00867.01 |
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| Immunogen | Recombinant GST protein (26 kD) |
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| Purification Method | Protein A affinity column |
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| Reconstitution | Reconstitute the lyophilized antibody (100 μg) with 100 μl of deionized water to a final antibody concentration of 1 mg/ml. |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Other applications: user-optimized |
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| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells |
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| Conjugation | Biotin |
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| Host Species | Mouse |
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| Note | Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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