| Catalog No. |
Size |
Price |
Figures |
A00873-40 ug
| 40 ug | $ 55.00 | MSDS: 20081118021403 (PDF)
EXAMPLE:
![Rabbit anti-KT3 polyclonal antibody [HRP]](http://www.genscript.com/site2/document/3691_20071227024121.JPG "Rabbit anti-KT3 polyclonal antibody [HRP]")
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A00873-200 ug
| 200 ug | $ 247.50 |
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| Full Name | | Rabbit Anti-KT3 [HRP] Polyclonal Antibody |
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| Alias | Rabbit Anti-KT3 [HRP] pAb |
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| Description | Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The KT3-Tag is an epitope tag composed of an 11-residue peptide, KPPTPPPEPET, derived from the carboxyl terminal sequence of simian virus 40 (SV40)'s large T-antigen. The GenScript Rabbit Anti-KT3-tag [HRP] Polyclonal Antibody is developed in rabbit hosts using a synthetic KT3-tag peptide conjugated to KLH.
GenScript Rabbit Anti-KT3 [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-KT3 [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain KT3-tags. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-KT3 [HRP] Polyclonal Antibody remains stable for 2-3 weeks if stored at 2-8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
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| Specificity | Rabbit Anti-KT3 [HRP] Polyclonal Antibody specifically binds to fusion proteins containing KT3 epitope tag. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
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| Immunogen | KT3 epitope tag peptide KPPTPPPEPET conjugated to KLH |
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| Purification Method | Immunoaffinity |
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized |
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| Conjugation | Horseradish peroxidase (HRP) |
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| Host Species | Rabbit |
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| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. |
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