| Catalog No. |
Size |
Price |
Figures |
A00874-40 ug
| 40 ug | $ 55.00 | EXAMPLE:
![Rabbit Anti-E pAb [HRP] Western Blot Analysis](http://www.genscript.com/site2/document/3632_20071206011025.JPG)
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|
|---|
A00874-200 ug
| 200 ug | $ 247.50 |
|---|
| Full Name | | Rabbit Anti-E-tag [HRP] Polyclonal Antibody |
|
| Alias | Rabbit Anti-E-tag [HRP] pAb |
|---|
| Description | GenScript Rabbit Anti-E-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-E-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E-tags. |
|---|
| Ig Subclass | Rabbit IgG
|
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| Storage | GenScript Rabbit Anti-E-tag [HRP] polyclonal antibody remains stable for 2-3 weeks if stored at 4°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
|---|
| Specificity | GenScript Rabbit-Anti-E specifically binds to over-expressed fusion proteins containing E epitope tags. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
|---|
| Immunogen | E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH |
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| Purification Method | Immunoaffinity chromatography
|
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| Applications | Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
|
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| Conjugation | Horseradish peroxidase (HRP) |
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| Host Species | Rabbit |
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| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.
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