| Catalog No. |
Size |
Price |
Figures |
A00877-40 ug
| 40 ug | $ 55.00 | EXAMPLE:
![Rabbit anti V5 pAb [HRP] Western Blot Analysis](http://www.genscript.com/site2/document/3634_20071206011610.JPG)
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A00877-200 ug
| 200 ug | $ 247.50 |
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| Full Name | | Rabbit Anti-V5-tag [HRP] Polyclonal Antibody |
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| Alias | Rabbit Anti-V5-tag [HRP] pAb |
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| Description | GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody remains stable for 2-3 weeks if stored at 4°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
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| Specificity | GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically binds to over-expressed fusion proteins containing V5 epitope tags. |
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| Concentration | 1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
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| Immunogen | V5 epitope tag peptide YTDIEMNRLGK conjugated to KLH |
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| Purification Method | Immunoaffinity |
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| Applications | The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
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| Conjugation | Horseradish peroxidase (HRP) |
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| Host Species | Rabbit |
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| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.
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