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Rabbit Anti-α-Actin-1 Polyclonal Antibody

Catalog No. Size Price Figures
A00885-40 ug
40 ug
$ 50.00
EXAMPLE:
Rabbit Anti-α-Actin-1 Polyclonal Antibody, one-step western blot analysis
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EXAMPLE:
Rabbit Anti-α-Actin-1 Polyclonal Antibody, western blot analysis
Zoom
A00885-200 ug
200 ug
$ 225.00
Full Name
Rabbit Anti-α-Actin-1 Polyclonal Antibody
AliasRabbit Anti-α-Actin-1 pAb
DescriptionThe two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.
GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide conjugated to KLH.
GenScript Rabbit Anti-α-Actin-1 Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.
Ig SubclassRabbit IgG
StorageGenScript Rabbit Anti-Alpha-Actin-1 remains stable for 2-3 weeks if stored at 4°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles.
SpecificityGenScript Rabbit Anti-Alpha-Actin-1 reacts with human, mouse, rat, cow, pig, rabbit, and some fish species. It has not yet been tested with other species.
Concentration0.5 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
ImmunogenSynthetic peptide GQVITIGNERFR conjugated to KLH
Purification MethodImmunoaffinity
ApplicationsWorking concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1.0 µg/ml
Western Blot Using the One-Step WesternTM Kit: For quick results, the GenScript one-Step WesternTM kit (L00204C) is recommended.
Mix 10 µg of GenScript Rabbit Anti-Alpha-Actin-1 with 10 ml of WB solution for any 8 cm X 8 cm membrane.
Other applications: user-optimized
ConjugationUnconjugated
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
* For Non-Clinical Research Use Only *

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