| |
| Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody |
|
|
| Catalog No. |
Size |
Price |
Figures |
A00896-40 ug
| 40 ug | $ 69.00 | EXAMPLE:
Zoom
References:
- Haque, N., et al. (1999) Brain Res. 838: 69-77.
- L Buee et al. (2000) Brain Res Brain Res Rev, 33(1):95-130.
- G.V.Johnson et al. (2004) J. Cell Sci. 117: 5721-5729.
|
|---|
A00896-200 ug
| 200 ug | $ 311.00 |
|---|
| Full Name | | Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody |
| | Alias | Rabbit Anti-Tau (Phospho-Thr217) pAb |
|---|
| Description | Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which range from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal part.
Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). These PHFs are also found in the lesions of other central nervous system disorders.
GenScript Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody is developed in rabbit using a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr217 of human Tau. |
|---|
| Ig Subclass | Rabbit IgG |
|---|
| Storage | GenScript Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody remains stable for 2-3 weeks if stored at 2-8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. |
|---|
| Specificity | GenScript Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody detects endogenous levels of Tau only when phosphorylated at threonine 217. |
|---|
| Concentration | 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide |
|---|
| Immunogen | Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr217 of human Tau |
|---|
| Purification Method | The antibody was purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide. |
|---|
| Applications | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized |
|---|
| Conjugation | Unconjugated |
|---|
| Species Reactivity | Human, mouse |
|---|
| Host Species | Rabbit |
|---|
| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
|---|
| * For Non-Clinical Research Use Only * If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
|
