Antibodies
You are here:  Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies  »  Tau Antibody (Phospho-Ser235), pAb, Rabbit   
 
  Search by Catalog Number:
   Search by Name:
   Search by Category:
 
Tau Antibody (Phospho-Ser235), pAb, Rabbit

Catalog No. Size Price Figures
A01096-40 ug
 		40 ug
$ 69.00
MSDS:  20080118034804  (PDF)
MSDS:  20081123201913  (PDF)

EXAMPLE:
Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody WB
Zoom
A01096-200 ug
 		200 ug
$ 311.00
Full Name
Tau Antibody (Phospho-Ser235), pAb, Rabbit
AliasRabbit Anti-Tau (Phospho-Ser235) pAb
DescriptionTau is a microtubule-associated phosphoprotein (MAP) localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms ranging in size from 352 to 441 amino acids. The tau variants differ from each other in the presence or absence of either three or four repeat-regions near the carboxy-terminal and the presence or absence of one or two inserts near the amino-terminal. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. Over thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404, and 413 and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein found in the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). These PHFs are also found in the lesions of other central nervous system disorders.
GenScript Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic phosphopeptide corresponding to the residues surrounding serine 235 of human tau.
Ig SubclassRabbit IgG
StorageThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityGenScript Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody detects endogenous levels of tau protein only when phosphorylated at serine 235.
Concentration0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
ImmunogenSynthetic phospho-peptide (KLH-coupled) corresponding to the residues surrounding phospho-serine 235 of human tau
Purification MethodAffinity chromatography using phospho-peptide, and cross-adsorbed with the corresponding non-phosphopeptide
ReconstitutionReconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
ApplicationsWorking concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized
ConjugationUnconjugated
Species ReactivityHuman, mouse. This product has not yet been tested with other species.
Host SpeciesRabbit
Antigen SpeciesHuman
NoteSmall volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.
Technical ManualTM0589, TM0590, TM0591, TM0592, TM0593
* For Non-Clinical Research Use Only *
Related Products:
CodeNameSizePrice 
A00402antibody : Tau Antibody (Phospho-Ser235), pAb, Rabbit40 ug
$ 69.00
200 ug
$ 311.00
A00393 antibody : Tau Antibody (Ab-235), pAb, Rabbit40 ug
$ 50.00
200 ug
$ 225.00
RP19726peptide : Tau (Phospho-Ser235) pAb Blocking Peptide100 ug
$ 50.00
RP19706 peptide : Tau (Ab-235) pAb Blocking Peptide100 ug
$ 50.00




1
 Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
 Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
 Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.



If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks.

Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance.