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Tau Antibody (Ser 262), pAb, Rabbit  |
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| Catalog No. |
Size |
Price |
Figures |
A01099-40 ug
| 40 ug | $ 50.00 | MSDS: 20081023031659 (PDF)
EXAMPLE:
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EXAMPLE:
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A01099-200 ug
| 200 ug | $ 225.00 |
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| Full Name | | Tau Antibody (Ser262), pAb, Rabbit |
| | Alias | Rabbit Anti-Tau (Ser262) pAb |
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| Description | Tau is a microtubule-associated phosphoprotein (MAP) which is localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms ranging from 352 to 441 amino acids. The tau variant differs from each other with the presence or absence of either three or four repeated regions near the carboxy terminal of the molecule and one or two inserts near the amino terminal.
Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein forming the paired helical filaments (PHFs), which further form the pathological neurofibrillary tangles leading to Alzheimer’s disease (AD). These PHFs are also found to be related to the lesions of other central nervous system disorders.
GenScript Rabbit Anti-Tau (Ser262) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide corresponding to the residues surrounding the serine 262 of human Tau. |
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| Ig Subclass | Rabbit IgG |
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| Storage | GenScript Rabbit Anti-Tau (Ser262) Polyclonal Antibody should be lyophilized for storage until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
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| Specificity | GenScript Rabbit Anti-Tau (Ser262) Polyclonal Antibody detects endogenous levels of total Tau protein. |
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| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
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| Immunogen | Synthetic peptide (KLH-coupled) corresponding to residues surrounding the serine 262 of human Tau |
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| Purification Method | Immunoaffinity chromatography |
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| Reconstitution | Reconstitute the lyophilized powder (40µg) with 80 µl of deionized water (or equivalent) to make the antibody concentration 0.5 mg/ml. |
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| Applications | Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized |
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| Conjugation | Unconjugated |
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| Species Reactivity | Human, mouse |
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| Host Species | Rabbit |
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| Note | Small volume of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
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| * For Non-Clinical Research Use Only *
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
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Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
If you cannot find your antibodies on our list, try our Express AntibodyTM Service, and receive high-quality, titer-guaranteed antibodies in only 5-8 weeks. Our customer service is available 24 hours, Monday through Friday. You may contact us any time for assistance. |
