| Cat. No. |
Size |
Price |
Figures |
|---|
RP10586-1 mg
| 1 mg | $ 33.25 | HPLC: 20060721145618 (PDF)
MS: 20060721145557 (PDF)
MSDS: 20080108225534 (PDF)
References:
- Gunawardane RN, et al. Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits. J. Cell Biol. Dec 2000; 151(7): 1513-1524.
- Park JH, Roeder RG. GAS41 Is Required for Repression of the p53 Tumor Suppressor Pathway during Normal Cellular Proliferation. Mol Cell Biol. Jun 2006; 26(11): 4006-4016.
- Herold MJ, et al. The stability and anti-apoptotic function of A1 are controlled by its C terminus. J Biol Chem. May 2006; 281(19): 13663-13671.
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|---|
| Full Name | |
| Alias |
DYKDDDDK tag; |
Sequence
(one-letter code) |
DYKDDDDK | Sequence
(three-letter code) | {ASP}{TYR}{LYS}{ASP}{ASP}{ASP}{ASP}{LYS} |
|---|
| Description | A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide. |
|---|
| Formula | C41H60N10O20 |
|---|
| M.W. | 1012.97 |
|---|
| Purity | > 95% |
|---|
| Storage | Store at 2-8°C. |
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