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Full Name
DYKDDDDK Peptide
Alias
DYKDDDDK tag;
Sequence (one-letter code)
DYKDDDDK
Sequence (three-letter code)
{ASP}{TYR}{LYS}{ASP}{ASP}{ASP}{ASP}{LYS}
Description
A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.
Formula
C41H60N10O20
M.W.
1012.97
Purity
> 95%
Storage
Store at 2-8°C.
* For Non-Clinical Research Use Only *
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