| Cat. No. |
Size |
Price |
Figures |
|---|
RP11737-1 mg
| 1 mg | $ 71.25 | HPLC: 20060721145623 (PDF)
MS: 20060721145602 (PDF)
MSDS: 20080919033928 (PDF)
References:
- Hammarstrom M, et al. Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein. J. Struct. Funct. Genomics. Jul 2006
- Woestenenk EA, et al. His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors. J. Struct. Funct. Genomics. 2004; 5(3): 217-229.
- Kuroda K and Ueda M. Bioadsorption of cadmium ion by cell surface-engineered yeasts displaying metallothionein and hexa-His. Appl. Microbiol. Biotechnol. Dec 2003; 63(2): 182-186.
|
|---|
RP11737-5 mg
| 5 mg | $ 190.00 |
|---|
| Full Name | |
| Alias |
His-tag, his tagged; epitope tag |
Sequence
(one-letter code) |
HHHHHH |
Sequence
(three-letter code) | {HIS}{HIS}{HIS}{HIS}{HIS}{HIS} |
|---|
| Description | The amino acid sequence consisting of 6 His residues in a row, called His-Tag, would act as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease - for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin. |
|---|
| Formula | C36H44N18O7
|
|---|
| M.W. | 840.85 |
|---|
| Purity | > 95% |
|---|
| Storage | Store at -20°C. Keep tightly closed. Store in a cool dry place |
