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GATA1 (Phospho-Ser142) pAb Blocking Peptide

Cat. No. Size Price Figures
RP19613-100 ug
 		100 ug
$ 50.00
MSDS:  20081008045928  (PDF)
PROTOCOL:  20100407015038  (PDF)

EXAMPLE:
Rabbit Anti GATA-1 (Phospho-Ser142) (polyclonal) Immunohistochemical analysis
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EXAMPLE:
Rabbit Anti GATA-1 (Phospho-Ser142) (polyclonal) Western Blot analysis
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EXAMPLE:
Rabbit Anti GATA-1 (Phospho-Ser142) (polyclonal) Immunofluorescence analysis
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Full Name
GATA1 (Phospho-Ser142) pAb Blocking Peptide
DescriptionThe synthesized phosphopeptide was derived from human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).
StorageStore at -20°C
Concentration100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol.
Quality ControlThe quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Applications

For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.

SpecificityThe peptide specifically blocks the signal of Rabbit Anti MEF2A (Ab-319) completely in western blotting and immunohistochemistry.
* For Non-Clinical Research Use Only *
Related Products:
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A00247antibody : GATA1 Antibody (Phospho-Ser142), pAb, Rabbit40 ug
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A00270 antibody : GATA1 Antibody (Ab-142), pAb, Rabbit40 ug
$ 50.00
200 ug
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 Peptide Modifications: GenScript offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
4
 Peptide Libraries: GenScript has developed a rapid high-throughput parallel peptide synthesis platform, providing custom libraries of unbound peptides of 5-20 mer at very competitive prices.




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