| Cat. No. |
Size |
Price |
Figures |
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RP19713-100 ug
| 100 ug | $ 50.00 | MSDS: 20081010031009 (PDF)
EXAMPLE:
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EXAMPLE:
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| Full Name | | p53 (Phospho-Ser315) pAb Blocking Peptide |
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| Description | The synthesized phosphopeptide was derived from human p53 around the phosphorylation site of serine 315 (S-S-SP-P-Q). |
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| Storage | Store at -20°C |
| Concentration | 100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol. |
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| Quality Control | The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. |
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| Applications | For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot. For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides. |
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| Specificity | The peptide specifically blocks the signal of Rabbit Anti p53 (Phospho-Ser315) completely in western blotting and immunohistochemistry.
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