For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.