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HSF1 (phospho-Ser303) pAb Blocking Peptide

Cat. No. Size Price Figures
RP19929-100 ug
 		100 ug
$ 50.00
EXAMPLE:
Immunohistochemical analysis using anti HSF1 (Phospho-Ser 303)
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EXAMPLE:
Western blot analysis using anti HSF1 (Phospho-Ser 303)
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Full Name
HSF1 (phospho-Ser303) pAb Blocking Peptide
DescriptionThe synthesized phosphopeptide was derived from human HSF1 around the phosphorylation site of serine 303 (P-P-SP-P-P).
StorageStore at -20°C
Concentration100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol.
Quality ControlThe quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Applications

For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.

SpecificityThe peptide specifically blocks the signal of Rabbit Anti HSF1 (phospho-Ser303) completely in Western blotting and immunohistochemistry.
* For Non-Clinical Research Use Only *
Related Products:
CodeNameSizePrice 
A00546antibody : Rabbit Anti-HSF1 (Phospho-Ser303) Polyclonal Antibody40 ug
$ 69.00
200 ug
$ 311.00
A00610 antibody : Rabbit Anti-HSF1 (Ab-303) Polyclonal Antibody40 ug
$ 50.00
200 ug
$ 225.00



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