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GAP43 (Ab-41) pAb Blocking Peptide

Cat. No. Size Price Figures
RP19954-100 ug
 		100 ug
$ 50.00
EXAMPLE:
Immunohistochemical analysis using GAP43 (Ab-41) Antibody
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EXAMPLE:
Western blot analysis using GAP43 (Ab-41) Antibody
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Full Name
GAP43 (Ab-41) pAb Blocking Peptide
DescriptionThe synthesized non-phosphopeptide was derived from human GAP43 around the phosphorylation site of serine 41 (Q-A-SP-F-R).
StorageStore at -20°C
Concentration100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol.
Quality ControlThe quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Applications

For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.

For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides.

SpecificityThe peptide specifically blocks the signal of GAP43 (Ab-41) antibody completely in Western blotting and immunohistochemistry.
* For Non-Clinical Research Use Only *

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