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| Hepatitis A Virus (HAV) VP1-P2A |
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| Catalog No. |
Size |
Price |
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Z00026-1 mg
| 1 mg | $ 420.00 |
References:
- Emerson SU, et al. Identification of VP1/2A and 2C as virulence genes of hepatitis A virus and demonstration of genetic instability of 2C. J. Virol. Sep 2002;76(17): 8551-8559.
- Kanda T, et al. Hepatitis A protein VP1-2A reduced cell viability in Huh-7 cells with hepatitis C virus subgenomic RNA replication. J. Gastroenterol Hepatol. Mar 2006; 21(3): 625-626.
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| Full Name | | Hepatitis A Virus (HAV) VP1-P2A |
| | Abbreviated Name-1 | HAV VP1-P2A; Hepatitis A Virus VP1-P2A; HAV VP1 |
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| Description | Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein and a panel of serum samples from acutely HAV-infected patients. The term 'antigenic domain' is used in this study to define a protein region spanned with consecutive overlapping immunoreactive peptides. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1,403-1,456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1,500-1,519 aa, includes almost the entire P3B, and the last domain, located at position 1,719-1,764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. Hepatitis A Virus (HAV) VP1-P2A is E. coli derived recombinant. The protein contains the VP1 –P2A immunodominant region. |
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| Source | E. coli |
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| Purity | Protein is >90% pure as determined by 10% PAGE (coomassie staining). |
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| Storage | Protein is shipped at ambient temperature. Upon arrival, store at -20°C. |
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| Concentration | 1 mg/ml in 10 mM CBB, pH 9.6, 0.1% SDS, 50% glycerol |
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| Stability | Five years frozen. one month in solution at room temperature. |
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| Specificity | Immunoreactive with sera of HAV-infected individuals |
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| Applications | Antigen in ELISA and Western blots, excellent antigen for detection of HAV with minimal specificity problems |
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