| Catalog No. |
Size |
Price |
Figures |
|---|
Z00054-100 ug
| 100 ug | $ 80.00 |
EXAMPLE:
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References:
- Shetty S, Idell S. Post-transcriptional regulation of urokinase mRNA. Identification of a novel urokinase mRNA-binding protein in human lung epithelial cells in vitro. J Biol Chem. May 2000; 275(18): 13771-13779.
- Hudson MA, McReynolds LM. Urokinase and the urokinase receptor: association with in vitro invasiveness of human bladder cancer cell lines. J Natl Cancer Inst. May 1997;89(10):709-717.
- Nienaber V, et al. Re-engineering of human urokinase provides a system for structure-based drug design at high resolution and reveals a novel structural subsite. J Biol Chem. Mar 2000; 275(10): 7239-7248.
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| Full Name | | | Abbreviated Name-1 | Urokinase |
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| Description | Urokinase (UK) is a serine protease, which is one of biological plasminogen activators. It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. It can be obtained from human urine or kidney cell culture. UK is a two-chain glycoprotein containing 411 amino acids with 12 dissulfide bonds. Its molecular weight is 54,000 Da. |
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| Source | Human urine |
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| M.W. | 54,000 Da |
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| Purity | Greater than 90.0% as determined by SDS-PAGE |
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| Specific Activity | 1 nM UK will cause a change in absorbance of 0.001 at 405 nm in 1 minute at R/T in 100 ul 0.05 M Tris-HCl, 0.1 M NaCl, pH 7.4, using S2444 (0.6 mM) as the substrate. The hUK conforms an activity greater than 68,000 IU/mg.
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| Storage | Lyophilized hUK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution hUK should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. |
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| Formulation | The hUK was lyophilized from a concentrated (1 mg/ml) solution with no additives. |
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| Reconstitution | It is recommended to reconstitute the lyophilized hUK in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Concentration | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of hUK as a Reference Standard.
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| Quality Control | Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II |
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