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| Full Name | |
| Abbreviated Name-1 | Disulfide Oxidoreductase |
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| Abbreviated Name-2 | DsbA |
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| Description | Disulfide Oxidoreductase (DsbA) (native) produced in E. coli is a periplasmic protein isolated from E. coli having a molecular mass of 23,149 Da. DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli. The DsbA is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 23,149 Da |
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| Purity | Greater than 95.0% as determined by the follwing:
((a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE |
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| Storage | Although lyophilized DsbA remains although stable at room temperature for three weeks, it's best stored desiccated below -18°C. Upon reconstitution, DsbA may be stored at 4°C for up to one week and below -18°C for longer periods. For long-term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles. |
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| Formulation | The protein is lyophilized from a sterile solution containing 50 mM sodium phosphate buffer and 100 mM sodium chloride. |
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| Reconstitution | It is recommended that reconstitute the lyophilized DsbA be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Applications | Suitable for use in Western Blot analysis. If this protein is to be used for Western Blot analysis, we recommend that the material be diluted in 1X SDS-PAGE sample buffer (1). On a 15-well minigel system, 50 ng of protein per lane should be sufficient when used in a colorimetric Western Blot with D9625 at a dilution of 1:10,000 as the primary antibody and an appropriate alkaline phosphatase conjugated secondary antibody for detection. |
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