| Catalog No. |
Size |
Price |
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| Full Name | |
| Abbreviated Name-1 | Cardiac Troponin C |
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| Abbreviated Name-2 | rHuTNC |
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| Description | Cardiac Troponin C (TNC), human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 18,020 Da.
The rHuTNC is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 18,020 Da |
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| Purity | Greater than 95.0% as determined by both:
(a) Analysis by RP-HPLC
(b) Anion-exchange FPLC
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuTNC |
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| Storage | rHuTNC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuTNC should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
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| Formulation | The protein solution (5.26 mg/ml) contains 150 mM NaCl, 10 mM sodium phosphate, 0.05% NaN3, pH 7.0. |
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| Reconstitution | It is recommended to reconstitute the lyophilized rHuTNC in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation was carried out by using 0.25-5.0 mg/ml Bradford assay vs. BSA |
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