| Catalog No. |
Size |
Price |
Figures |
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Z00327-10 ug
| 10 ug | $ 95.00 | MSDS: 20111226200555 (PDF)
COA: Z00327_13165701 (PDF)
References:
- Zhao M, et al. The zinc finger transcription factor gli2 mediates bone morphogenetic protein 2 expression in osteoblasts in response to hedgehog signaling. Mol. Cell Biol. Aug 2006; 26(16): 6197-6208.
- Hoshino M, et al. Repair of long intercalated rib defects using porous beta-tricalcium phosphate cylinders containing recombinant human bone morphogenetic protein-2 in dogs. Biomaterials. Oct 2006; 27(28): 4934-4940.
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Z00327-50 ug
| 50 ug | $ 237.00 |
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Z00327-1 mg
| 1 mg | $ 1185.00 |
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| Full Name | | Bone Morphogenetic Protein-2 (BMP-2), human |
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| Abbreviated Name-1 | rHuBMP-2 |
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| Abbreviated Name-2 | Bone Morphogenetic Protein2; BMP-2; BMP2 |
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| Description | Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and a member of the transforming growth factor-β (TGF-β) superfamily of proteins. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or as a heterodimer of two related bone morphogenetic proteins.
Recombinant human Bone Morphogenetic Protein-2 (BMP-2) produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 26,018 Da. Human Bone Morphogenetic Protein-2 (BMP-2) is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 26,018 Da |
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| Purity | Greater than 95.0% as determined by the following methods:
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel |
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| Endotoxin Level | Less than 0.1 ng/μg (1 IEU/μg) of human Bone Morphogenetic Protein-2 (BMP-2) |
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| Specific Activity | Recombinant human Bone Morphogenetic Protein-2 (BMP-2) is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml corresponding to a specific activity of 2.0 x 104 IU/mg. |
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| Storage | Lyophilized recombinant human Bone Morphogenetic Protein-2 (BMP-2) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution rHuBMP-2 should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles. |
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| Formulation | Human Bone Morphogenetic Protein-2 (BMP-2) was lyophilized after extensive dialysis against 50mM Acetic acid. |
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| Reconstitution | It is recommended that the lyophilized human Bone Morphogenetic Protein-2 (BMP-2) be reconstituted in sterile 20 mM HAc (acetic acid) not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
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| Sequence analysis | The sequence of the first five N-terminal amino acids has been found to be Met-Gln-Ala-Lys-His. |
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