| Catalog No. |
Size |
Price |
Figures |
|---|
Z00333-10 ug
| 10 ug | $ 26.00 |
STRUCTURE:
Zoom
References:
- Katagiri S, Takahashi Y. Potential relationship between normalization of endometrial epidermal growth factor profile and restoration of fertility in repeat breeder cows. Anim. Reprod. Sci. Sep 2006; 95(1-2): 54-66.
- Endo K, et al. Evaluation of the epidermal growth factor receptor gene mutation and copy number in non-small cell lung cancer with gefitinib therapy. Oncol. Rep. Sep 2006; 16(3): 533-541.
|
|---|
Z00333-50 ug
| 50 ug | $ 80.00 |
|---|
Z00333-1 mg
| 1 mg | $ 180.00 |
|---|
| Full Name | | Epidermal Growth Factor (EGF), human |
|
| Abbreviated Name-1 | rHuEGF |
|---|
| Abbreviated Name-2 | Epidermal Growth Factor |
|---|
| Description | Epidermal Growth Factor (EGF), human is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6,200 Da protein containing 53 amino acid residues. |
|---|
| Source | E. coli |
|---|
| M.W. | 6.0 kDa+/-10% determined by reduced SDS-PAGE |
|---|
| Isoelectric Point | The main zone between 4.0-5.0 analysis by IEF |
|---|
| UV Scan | The maximal absorption wave is 275+/-3 nm. |
|---|
| Purity | Greater than 95% as determined by
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
|---|
| Endotoxin Level | Less than 0.3 ng/μg (0.3 IEU/μg) determined by LAL test |
|---|
| Specific Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/ mg. |
|---|
| Storage | Lyophilized rHuEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuEGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
|---|
| Formulation | The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives. |
|---|
| Reconstitution | It is recommended to reconstitute the lyophilized rHuEGF in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
|---|
| Quantitation | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard. |
|---|
| Sequence | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR |
|---|
| Sequence analysis | The sequence of the first fifteen N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys-Leu.
|
|---|
