| Catalog No. |
Size |
Price |
Figures |
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Z00339-10 ug
| 10 ug | $ 70.00 |
References:
- Skaletz-Rorowski A, et al. Angiotensin AT1 receptor upregulates expression of basic fibroblast growth factor, basic fibroblast growth factor receptor and coreceptor in human coronary smooth muscle cells. Basic Res. Cardiol. Jul 2004; 99 (4): 272-278.
- Dosanjh A, et al. Respiratory syncytial virus augments production of fibroblast growth factor basic in vitro: implications for a possible mechanism of prolonged wheezing after infection. Pediatr. Allergy. Immunol. Dec 2003; 14 (6): 437-440.
- Skaletz-Rorowski A, et al. Heparin-induced overexpression of basic fibroblast growth factor, basic fibroblast growth factor receptor, and cell-associated proteoheparan sulfate in cultured coronary smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. Aug 1996; 16 (8): 1063-1069.
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| Full Name | | Fibroblast Growth Factor (FGF)-basic, human |
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| Abbreviated Name-1 | rHuFGF-b |
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| Abbreviated Name-2 | Fibroblast growth factor-basic |
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| Description | GenScript Fibroblast Growth Factor (FGF)-basic, human produced in E. coli, is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17,353 Da. The rHuFGF-b is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 17,353 Da |
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| Cas | 106096-93-9 |
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| Purity | Greater than 98.0% as determined by the following:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuFGF-b |
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| Specific Activity | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is 2×106 units/mg. |
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| Storage | Lyophilized rHuFGF-b although stable at room temperature for up to three weeks, should be stored desiccated at -18°C or below. Upon reconstitution, rHuFGF-b may be stored at 4°C for short periods (two to seven days), but is best stored at -18°C or below for longer periods. For long-term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles. |
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| Formulation | The protein is lyophilized from a concentrated (1 mg/ml) sterile solution containing 5 mM Tris pH 7.5 and 150 mM NaCl. |
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| Reconstitution | It is recommended that the lyophilized rHuFGF-b be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation is carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis using a calibrated solution of FGF-b as a reference standard. |
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| Sequence | AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS |
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and has been found to be Ala-Ala-Gly-Ser-Ile. |
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