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| Full Name | |
| Abbreviated Name-1 | rHuHGF |
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| Abbreviated Name-2 | Hepatocyte Growth Factor |
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| Description | GenScript Hepatocyte Growth Factor (HGF), human produced in Baculovirus， is a heterodimer, non-glycosylated, polypeptide chain having a total molecular mass of 80,017 Da. |
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| Source | Baculovirus Infected High-5 cells |
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| M.W. | 80,017 Da |
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| Purity | Greater than 98.0% as determined by the following methods:
(a) RP-HPLC analysis
(b) Anion-exchange FPLC
(c) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuHGF. |
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| Specific Activity | The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells has been found to be 20-40 ng/ml. |
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| Storage | Although lyophilized rHuHGF remains stable at room temperature for three weeks, it is best stored desiccated below -18°C. Upon reconstitution, rHuHGF may be stored at 4°C for up to one week and below -18°C for longer periods. For long-term storage it is recommended that a carrier protein (0.1% HSA or BSA)be added. Aviod freeze-thaw cycles. |
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| Formulation | The protein is lyophilized from a concentrated solution containing 0.1 M L-Arginine and 10 mM Tris at pH8. |
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| Reconstitution | It is that the lyophilized rHuHGF be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation is carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)
2. RP-HPLC analysis, using a calibrated solution of HGF as a reference standard |
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| Sequence | alpha chain QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR
beta chain VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS
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| Sequence analysis | Agrees with the sequence of native human HGF |
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