| Catalog No. |
Size |
Price |
Figures |
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Z00353-10 ug
| 10 ug | $ 26.00 |
References:
- Siebeck N, et al. Effects of human recombinant alpha-2b interferon and feline recombinant omega interferon on in vitro replication of feline herpesvirus-1. Am. J. Vet. Res. Aug 2006; 67 (8): 1406-1411.
- Abukawa H, et al. Effect of interferon-alpha-2b on porcine mesenchymal stem cells. J. Oral Maxillofac. Surg. Aug 2006; 64 (8): 1214-1220.
- Yang L, et al. A novel method to prepare highly encapsulated interferon-alpha-2b containing liposomes for intramuscular sustained release. Eur. J. Pharm. Biopharm. Aug 2006; 64 (1): 9-15.
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Z00353-50 ug
| 50 ug | $ 80.00 |
|---|
Z00353-1 mg
| 1 mg | $ 480.00 |
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| Full Name | | Interferon (IFN)-α 2b, human |
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| Abbreviated Name-1 | rHuIFN-α-2b; rHuIFNα-2b; rHuIFN-a-2b; rHuIFN-alfa-2b; rHuIFNalfa-2b |
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| Abbreviated Name-2 | Interferon-α-2b; Interferonα-2b; Interferon-a-2b; Interferon-alfa-2b; Interferonalfa-2b |
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| Description | GenScript Interferon (IFN)-α 2b, human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Da. The Interferon-alpha2b gene was obtained from human leukocytes. The rHuIFN-α 2b is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 19,269 Da |
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| Isoelectric Point | The main zone between 5.5-6.8 analysis by IEF. |
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| UV Scan | The maximal absorption wave is 278+/-3 nm. |
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| Purity | Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuIFN-α 2b |
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| Specific Activity | The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6×108/mg. |
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| Storage | Lyophilized rHuIFN-α 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIFN-α 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles. |
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| Formulation | Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer. |
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| Reconstitution | It is recommended to reconstitute the lyophilized rHuIFN-α 2b in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2b as a Reference Standard. |
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln. N-terminal methionine has been completely removed enzymatically.
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