| Catalog No. |
Size |
Price |
Figures |
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Z00358-10 ug
| 10 ug | $ 26.00 |
References:
- Jo YW, et al. Long-acting interferon-alpha 2a modified with a trimer-structured polyethylene glycol: preparation, in vitro bioactivity, in vivo stability and pharmacokinetics. Int. J. Pharm. Feb 2006; 309 (1-2): 87-93.
- Asik Akman S, et al. Preliminary results of interferon alpha-2a and lamivudine combination therapy regimen in children with chronic hepatitis B. Mikrobiyol. Bul. Jan-Apr 2006; 40 (1-2): 47-53.
- Ajariyakhajorn C, et al. Randomized, placebo-controlled trial of nonpegylated and pegylated forms of recombinant human alpha interferon 2a for suppression of dengue virus viremia in rhesus monkeys. Antimicrob. Agents Chemother. Nov 2005; 49 (11): 4508-4514.
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Z00358-50 ug
| 50 ug | $ 80.00 |
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Z00358-1 mg
| 1 mg | $ 480.00 |
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| Full Name | | Interferon (IFN)-α 2a, human |
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| Abbreviated Name-1 | rHuIFN-α 2a; rHuIFNα 2a; rHuIFN-a 2a; rHuIFN-alfa 2a; rHuIFNalfa 2a |
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| Abbreviated Name-2 | Interferon-α 2a; Interferonα 2a; Interferon-a 2a; Interferon-alfa 2a; Interferonalfa 2a |
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| Description | GenScript Interferon (IFN)-α 2a, human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,241 Da. The Interferon-alpha 2a gene was obtained from human leukocytes. The rHuIFN-alpha 2a is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 19,241 Da |
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| Purity | Greater than 98.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuIFN-α 2a |
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| Specific Activity | The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7×108 IU/mg. |
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| Storage | Lyophilized rHuIFN-alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIFN-α 2a should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles. |
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| Formulation | Lyophilized from (1 mg/ml) solution containing 7.21 sodium chloride and 0.77 mg ammonium acetate. |
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| Reconstitution | It is recommended to reconstitute the lyophilized rHuIFN-α 2a in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-α 2a as a Reference Standard.
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-α. N-terminal methionine has been completely removed enzymatically.
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