| Catalog No. |
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| Full Name | |
| Abbreviated Name-1 | rHuIL-4; rHuIL4; IL-4; IL4 |
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| Abbreviated Name-2 | Human Interleukin-4; Human Interleukin4 |
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| Description | Recombinant human IL-4, produced in E.coli, is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15,000 Da. The rHuIL-4 is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 15,000 Da |
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| Purity | Greater than 98.0% as determined by the following methods:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuIL-4 |
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| Specific Activity | The ED50, as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a specific activity of 5×106 IU/mg. |
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| Storage | Although lyophilized rHuIL-4 remains stable at room temperature for three weeks, it's best stored desiccated below -18°C. Upon reconstitution rHuIL-4 may be stored at 4°C for up to one week and below -18°C for longer periods. For long term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid freeze-thaw cycles. |
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| Formulation | Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives |
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| Reconstitution | It is recommended that the lyophilized rHuIL-4 be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation is carried out by two independent methods:
1. UV spectroscopy at 280 nm uses the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program (IntelliGenetics).
2. RP-HPLC analysis using a standard solution of IL-4 as a reference standard |
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| Sequence | MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS |
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| Sequence analysis | The sequence of the first five N-terminal amino acids has been found to be Met-His-Lys-Cys-Asp.
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