| Catalog No. |
Size |
Price |
Figures |
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Z00372-10 ug
| 10 ug | $ 91.00 |
References:
- Wernstedt I, et al. Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006
- Itoh S, et al. A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006; 39 (3): 505-512.
- Pang Y, et al. Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006; 141 (2): 745-755.
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Z00372-50 ug
| 50 ug | $ 300.00 |
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Z00372-1 mg
| 1 mg | $ 1600.00 |
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| Full Name | | Interleukin (IL)-6, human |
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| Abbreviated Name-1 | rHuIL-6; rHuIL6 |
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| Abbreviated Name-2 | Interleukin-6; Interleukin6 |
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| Description | Interleukin-6 is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-β-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. GenScript Interleukin (IL)-6, human produced in E. coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,000 Da. The rHuIL-6 is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 21,000 Da |
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| Purity | Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Anion-exchange FPLC
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
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| Endotoxin Level | Less than 0.1 ng/μg (IEU/μg) of rHuIL-6 |
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| Specific Activity | The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg. |
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| Storage | Lyophilized rHuIL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
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| Formulation | Each μg of rHuIL-6 contains 43 μg sodium chloride and 50 μg HSA |
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| Reconstitution | It is recommended to reconstitute the lyophilized rHuIL-6 in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard. |
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| Sequence | PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM |
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
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