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Interleukin-6 (IL-6), human

Catalog No. Size Price Figures
Z00372-10 ug
10 ug
$ 91.00


References:
  • Wernstedt I, et al. Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006
  • Itoh S, et al. A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006; 39 (3): 505-512.
  • Pang Y, et al. Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006; 141 (2): 745-755.
Z00372-50 ug
50 ug
$ 300.00
Z00372-1 mg
1 mg
$ 1600.00
Full Name
Interleukin-6 (IL-6), human
Abbreviated Name-1rHuIL-6; rHuIL6
Abbreviated Name-2Interleukin-6; Interleukin6
DescriptionInterleukin-6 is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-β-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. GenScript Interleukin (IL)-6, human produced in E. coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,000 Da. The rHuIL-6 is purified by proprietary chromatographic techniques.
SourceE. coli
M.W.21,000 Da
PurityGreater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Anion-exchange FPLC
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Endotoxin LevelLess than 0.1 ng/μg (IEU/μg) of rHuIL-6
Specific ActivityThe ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg.
StorageLyophilized rHuIL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
FormulationEach μg of rHuIL-6 contains 43 μg sodium chloride and 50 μg HSA
ReconstitutionIt is recommended to reconstitute the lyophilized rHuIL-6 in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
QuantitationProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
SequencePVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Sequence analysisThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
* For Non-Clinical Research Use Only *
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