| Catalog No. |
Size |
Price |
Figures |
|---|
Z00374-10 ug
| 10 ug | $ 91.00 |
References:
- Mege JL, et al. The two faces of interleukin 10 in human infectious diseases. Lancet Infect. Dis. Sep 2006; 6(9): 557-569.
- Konno S, et al. Interleukin-10 and Th2 cytokines differentially regulate osteopontin expression in human monocytes and dendritic cells. J. Interferon Cytokine Res. 2006 Aug; 26(8): 562-567.
- Kamali-Sarvestani E, et al. Association study of IL-10 and IFN-gamma gene polymorphisms in Iranian women with preeclampsia. J. Reprod. Immunol. Jul 2006. 16863661
|
|---|
Z00374-50 ug
| 50 ug | $ 300.00 |
|---|
Z00374-1 mg
| 1 mg | $ 1600.00 |
|---|
| Full Name | | Interleukin (IL)-10, human |
|
| Abbreviated Name-1 | rHuIL-10; rHuIL10; IL-10; IL10 |
|---|
| Abbreviated Name-2 | Human Interleukin-10; Human Interleukin10 |
|---|
| Description | Recombinant Human IL-10 produced in CHO is a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18,648 Da. The rHuIL-10 is purified by proprietary chromatographic techniques. |
|---|
| Source | E. coli |
|---|
| M.W. | 18,648 Da |
|---|
| Purity | Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
|---|
| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuIL-10. |
|---|
| Specific Activity | The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2 ng/ml, corresponding to a Specific Activity of 1.5×106 IU/mg. |
|---|
| Storage | Lyophilized rHuIL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles. |
|---|
| Formulation | The protein was lyophilized from a concentrated (1 mg/ml) solution containing 60 mM PBS pH=6.5 and 100 mM NaCl, |
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| Reconstitution | It is recommended to reconstitute the lyophilized rHuIL-10 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr. |
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