| Catalog No. |
Size |
Price |
Figures |
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Z00377-10 ug
| 10 ug | $ 91.00 |
References:
- Suzuki A, et al. Interleukin-15 increases hepatic regenerative activity. J. Hepatol. Sep 2006; 45 (3): 410-418.
- Lin SJ, et al. Effect of interleukin-15 on alloreactivity in umbilical cord blood. Transpl. Immunol. Aug 2006; 16 (2): 112-116.
- Waldmann TA. The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat. Rev. Immunol. Aug 2006; 6 (8): 595-601.
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Z00377-50 ug
| 50 ug | $ 320.00 |
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Z00377-1 mg
| 1 mg | $ 1600.00 |
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| Full Name | | Interleukin (IL)-15, human |
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| Abbreviated Name-1 | rHuIL-15; rHuIL15 |
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| Abbreviated Name-2 | Interleukin-15; Interleukin15 |
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| Description | Interleukin-15 (IL-15) is a cytokine that possesses a variety of biological functions. IL-15 stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R. Recombinant Human IL-15 produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,774 Da. |
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| Source | E. coli |
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| M.W. | 12,774 Da |
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| Purity | Greater than 98.0% as determined by:
(a)Analysis by RP-HPLC
(b)Anion-exchange FPLC
(c)Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuIL-15 |
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| Specific Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be <0.5 ng/ml, corresponding to a Specific Activity of 2×106 IU/mg. |
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| Storage | Lyophilized rHuIL-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles. |
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| Formulation | The protein was lyophilized from a concentrated (1 mg/ml) solution with 5 mM Tris pH 8.0. |
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| Reconstitution | It is recommended to reconstitute the lyophilized rHuIL-15 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard. |
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| Sequence | MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS |
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val.
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