| Catalog No. |
Size |
Price |
Figures |
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Z00384-10 ug
| 10 ug | $ 140.00 |
References:
- Yu C, et al. TNF reduces LIF endocytosis despite increasing NFkappaB-mediated gp130 expression. J. Cell. Physiol. Oct 2007; 213(1): 161-6.
- Fayon M, et al. Increased secretion of leukemia inhibitory factor by immature airway smooth muscle cells enhances intracellular signaling and airway contractility. Am. J. Physiol. Lung Cell Mol. Physiol. Aug 2006; 291(2): L244-L251.
- McColm JR, et al. Exogenous leukemia inhibitory factor (LIF) attenuates retinal vascularization reducing cell proliferation not apoptosis. Exp. Eye Res. Aug 2006; 83(2): 438-446.
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Z00384-50 ug
| 50 ug | $ 400.00 |
|---|
Z00384-1 mg
| 1 mg | $ 3120.00 |
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| Full Name | | Leukemia Inhibitory Factor (LIF), human |
|
| Abbreviated Name-1 | rHuLIF |
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| Abbreviated Name-2 | Leukemia Inhibitory Factor |
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| Description | Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, stem cell pluripotency control, bone and fat metabolism, mitogenesis of certain factor-dependent cell lines, and the promotion of megakaryocyte production in vivo. Mature human and murine mature LIFs exhibit 78% sequence identity at the amino acid control. Human LIF is equally active on both human and mouse cells. Murine LIF is approximately 1000 times less active on human cells than hLIF. Recombinant Human LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19,717 Da. |
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| Source | E. coli |
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| M.W. | 19,717 Da |
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| Purity | Greater than 95% as determined by the following methods:
(a) RP-HPLC analysis
(b) Anion-exchange FPLC
(c) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
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| Endotoxin Level | Less than 0.1 ng/ug (1 IEU/ug) of LIF |
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| Specific Activity | The ED50 is determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of > 1×108 units/mg. |
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| Storage | Lyophilized rHuLIF remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution rHuLIF should be stored at 4°C for up to one week and -18°C for longer periods it is recommended that a carrier protein (0.1% HSA or BSA) be added. Please avoid repeated freeze-thaw cycles
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| Formulation | LIF is lyophilized after extensive dialysis against PBS. |
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| Reconstitution | We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. We also recommend that further dilutions be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added. This solution can then be stored at 4°C for one week or at -20°C for longer periods. |
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| Sequence analysis | The sequence of the first five N-terminal amino acids has been found to be Ser-Pro-Leu-Pro-Ile.
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