| Catalog No. |
Size |
Price |
Figures |
|---|
Z00393-10 ug
| 10 ug | $ 170.00 |
References:
- de Haan G, et al. Efficient mobilization of haematopoietic progenitors after a single injection of pegylated recombinant human granulocyte colony-stimulating factor in mouse strains with distinct marrow-cell pool sizes. Br. J. Haematol. Sep 2000; 110(3): 638-646.
- Sainathan SK, et al. PEGylated murine Granulocyte-macrophage colony-stimulating factor: production, purification, and characterization. Protein Expr. Purif. Dec 2005; 44(2): 94-103.
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Z00393-50 ug
| 50 ug | $ 640.00 |
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Z00393-1 mg
| 1 mg | $ 3200.00 |
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| Full Name | | Pegylated Granulocyte Colony Stimulating Factor (PEG-G-CSF), human |
|
| Abbreviated Name-1 | rHuPEG-G-CSF |
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| Abbreviated Name-2 | PEG-G-CSF |
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| Description | GenScript Pegylated Granulocyte Colony Stimulating Factor (PEG-G-CSF), human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da. PEG-GCSF is manufactured by attaching a 20,000 Da methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of G-CSF having a total molecular mass of 38,800 Da. |
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| Source | E. coli |
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| M.W. | 20,000 Da |
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| Purity | Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel. |
|---|
| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuPEG-G-CSF. |
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| Specific Activity | The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 1×108 IU/mg. |
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| Storage | rHuPEG-G-CSF although stable at 15°C for 1 week should be stored between 2°C-8°C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA). |
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| Formulation | The protein was extensive dialyzed against 10 mM sodium acetate buffer PH= 4 and 5% mannitol was added. |
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| Quantitation | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction
coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE
computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of PEG-G-CSF as a Reference Standard.
|
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly. |
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