| Catalog No. |
Size |
Price |
Figures |
|---|
Z00394-20 ug
| 20 ug | $ 350.00 |
References:
- Sporea I, et al. Pegylated-interferon alpha 2a treatment for chronic hepatitis C in patients on chronic haemodialysis. World J. Gastroenterol. Jul 2006; 12 (26): 4191-4194.
- Yenice N, et al. The efficacy of pegylated interferon alpha 2a or 2b plus ribavirin in chronic hepatitis C patients. Turk. J. Gastroenterol. Jun 2006; 17 (2): 94-98.
- Kiladjian JJ, et al. High molecular response rate of polycythemia vera patients treated with pegylated interferon alpha-2a. Blood. May 2006
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Z00394-100 ug
| 100 ug | $ 700.00 |
|---|
Z00394-1 mg
| 1 mg | $ 3960.00 |
|---|
| Full Name | | Pegylated Interferon (PEG-IFN)-α 2a, human |
|
| Abbreviated Name-1 | rHuPEG-IFN-α 2a; rHuPEG-IFNα 2a; rHuPEG-IFN-a 2a; rHuPEG-IFN-alfa 2a; rHuPEG-IFNalfa 2a |
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| Abbreviated Name-2 | Pegylated Interferon-α 2a; Pegylated Interferonα 2a; Pegylated Interferon-a 2a; Pegylated Interferon-alfa 2a |
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| Description | GenScript Pegylated Interferon (PEG-IFN)-α 2a, human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,241 Da. The Interferon-α 2a gene was obtained from human leukocytes. rHuPEG-IFN-α 2a is manufactured by attaching a 40,000 Da methoxypolyethylene glycol propionaldehyde (mPEG-ALD) with a total molecular mass of 59,241 Da to the N-terminal amino acid of IFN-α 2a. The rHuIFN-α 2a is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 19,241 Da |
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| Purity | Greater than 98.0% as determined by the following methods:
(a) RP-HPLC analysis
(b) Anion-exchange FPLC
(c) Reducing and non-reducing SDS-PAGE Silver Stained gel analysis |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuPEG-IFN-α 2a. |
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| Specific Activity | The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells is 0.7 X 108. |
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| Storage | rHuPEG-IFN-α 2a should be stored between 4°C and 8°C. For long-term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. |
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| Formulation | The protein 0.18 mg/ml solution contains 8 mg sodium chloride, 0.05 mg tween 80, 0.05 mg acetic acid, and 2.62 mg sodium acetate pH=6. |
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| Quantitation | Protein quantitation is carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis using a calibrated solution of IFN- α 2a as a reference standard.
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| Sequence analysis | The sequence of the first five N-terminal amino acids has been found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-alpha. The N-terminal methionine has been completely removed enzymatically.
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