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| Full Name | |
| Abbreviated Name-1 | rHuTPO |
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| Abbreviated Name-2 | Thrombopoietin |
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| Description | GenScript Thrombopoietin (TPO), human produced in E. coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18,608 Da, which comprises the receptor binding domain of the Mpl-ligand protein. The rHuTPO is purified by proprietary chromatographic techniques. |
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| Source | E. coli |
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| M.W. | 18,608 Da |
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| Purity | Greater than 98.0% as determined by the following methods:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rHuTPO |
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| Specific Activity | The ED50 as determined by the dose-dependant stimulation of MO7e cells is < 1 ng/ml, corresponding to a specific activity of 1×106 IU/mg. |
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| Storage | Although lyophilized rHuTPO can remain stable at room temperature for three weeks, it is best stored desiccated below -18°C. Upon reconstitution, rHuTPO may be stored at 4°C for up to one week and below -18°C for longer periods. For long-term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeat freeze-thaw cycles. |
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| Formulation | Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives |
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| Reconstitution | It is recommended that the lyophilized rHuTPO be reconstituted in sterile 18 MΩ-cm H2O of not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm
2. RP-HPLC analysis, using a standard solution of IL-3 as a reference standard
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| Sequence | SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL |
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| Sequence analysis | The sequence of the first five N-terminal amino acids has been found to be Ser-Pro-Ala-Pro-Pro. |
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