| Catalog No. |
Size |
Price |
Figures |
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Z00414-2 ug
| 2 ug | $ 70.00 |
References:
- Candolfi M, Jaita G et al. Adenoviral vectors encoding tumor necrosis factor-alpha and FasL induce apoptosis of normal and tumoral anterior pituitary cells. J Endocrinol. Jun 2006;189(3):681-90.
- Szlosarek PW, Grimshaw MJ et al. Expression and regulation of tumor necrosis factor alpha in normal and malignant ovarian epithelium. Mol Cancer Ther. Feb 2006;5(2):382-90.
- Lean IS, Lacroix-Lamande S et al. Role of tumor necrosis factor alpha in development of immunity against Cryptosporidium parvum infection. Infect Immun. Jul 2006;74(7):4379-82.
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| Full Name | | Tumor Necrosis Factor (TNF)-α, murine |
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| Abbreviated Name-1 | TNF-α; TNFα; TNF-a; TNFa; TNF-alpha; TNFalpha |
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| Abbreviated Name-2 | Tumor Necrosis Factorα; Tumor Necrosis Factor-a; Tumor Necrosis Factora; Tumor Necrosis Factor-alpha; Tumor Necrosis Factoralpha |
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| Description | Recombinant murine TNF-alpha produced in S. lividans is a single, glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17,301.32 Da. rmTNF-alpha is purified by standard chromatographic techniques. |
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| Source | Streptomyces lividans |
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| M.W. | 17,301.32 Da |
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| Purity | Greater than 98.0% as determined by the following:
(a) Analysis by RP-HPLC
(c) Analysis by SDS-PAGE |
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| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of rmTNF-alpha |
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| Specific Activity | The product shows itself fully biologically active when compared to standard. Its ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of 9×108 IU/mg. |
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| Storage | Lyophilized rmTNF-alpha although stable at room temperature for three weeks, may be stored desiccated below -18°C. Upon reconstitution rmTNF-alpha should be stored at 4°C for up to seven days and for longer periods -18°C. It is also recommended that a carrier protein (0.1% HSA or BSA) be added for long-term storage. Avoid repeated freeze-thaw cycles. |
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| Formulation | The protein is lyophilized from a concentrated (1 mg/ml) sterile solution containing 20 mM Tris-HCL, pH=8, 5 mM MgCl2 and 10% glycerol. |
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| Reconstitution | It is recommended that to reconstitute the lyophilized rmTNF-alpha be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Quantitation | Protein quantitation was carried out by the following two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HP analysis
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| Sequence | MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
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| Sequence analysis | The sequence of the first five N-terminal amino acids has been found to be Met-Leu-Arg-Ser-Ser. |
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