| Catalog No. |
Size |
Price |
Figures |
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Z01001-10 ug
| 10 ug | $ 52.00 |
References:
- Angelini DJ, et al. TNF{alpha} Increases Tyrosine Phosphorylation of Vascular Endothelial-Cadherin and Opens the Paracellular Pathway Through Fyn Activation in Human Lung Endothelia. Am. J. Physiol. Lung Cell Mol. Physiol. Aug 2006.
- Angelone DF, et al. Innate Immunity of the Human Newborn Is Polarized Toward a High Ratio of IL-6/TNF-{alpha} Production In Vitro and In Vivo. Pediatr. Res. Jun 2006.
- Frankel SK, et al. TNF-alpha sensitizes normal and fibrotic human lung fibroblasts to Fas-induced apoptosis. Am. J. Respir. Cell Mol. Biol. Mar 2006; 34(3): 293-304.
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Z01001-50 ug
| 50 ug | $ 129.00 |
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Z01001-1 mg
| 1 mg | $ 1050.00 |
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| Full Name | | Tumor Necrosis Factor (TNF)-α, human |
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| Abbreviated Name-1 | Human tumor necrosis factor alpha; Human tumor necrosis factor alfa; Human tumor necrosis factor-alpha; Human tumor necrosis factor-alfa; Human tumor necrosis factor a |
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| Abbreviated Name-2 | TNF-a; TNFa; TNFα; TNF-α; TNF-alpha; TNFalpha; TNF alpha; TNF-alfa; TNFalfa; TNF alfa |
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| Description | TNF-α is an homotrimer with a subunit molecular mass of 17,000 Da. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.
Tumor necrosis factor alpha is a non-glycosylated cytokine produced from E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17,484 Da. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. |
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| Source | E. coli |
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| M.W. | 17,300 Da+/-10% determined by reduced SDS-PAGE |
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| Isoelectric Point | The main zone between 4.0-5.0 analysis by IEF |
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| UV Scan | The maximal absorption wave is 275+/-3 nm. |
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| Purity | Greater than 95% as determined by:
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel |
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| Endotoxin Level | Less than 0.01 ng/µg (0.01 IEU/μg) determined by LAL test |
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| Specific Activity | The specific activity has been found to be greater than 3.0×107 IU/mg. |
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| Storage | Lyophilized samples are stable for up to 12 months from date of delivery if stored between -20°C and -70°C. Avoid repeated freeze-thaw cycles. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS |
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| Reconstitution | It is recommended that the lyophilized TNF-α be reconstituted in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
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| Quality Control | Residual DNA: less than 10 ng/mg, analysis by solid phase blot
Residual host cell protein: less than 0.1%, analysis by ELISA |
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| Sequence | MVRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL |
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| Sequence analysis | The sequence of the first fifteen N-terminal amino acids has been found to be Val-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser-Asp-Lys-Pro-Val-Ala-His.
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