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Enterokinase, Light Chain, Porcine

Catalog No. Size Price Figures
Z01003-100 IU
 		100 IU
$ 80.00
MSDS:  20060502233525  (PDF)
PROTOCOL:  20061122042342  (PDF)
COA:  Z01003_R30071001  (PDF)
COA:  Z01003_R30071002  (PDF)
COA:  Z01003_R30061003  (PDF)

EXAMPLE:
Digestion of Thioredoxin-NP-27 fusion protein with Porcine EK
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References:
  • Palmai-Pallag T, et al. The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins. FEBS J. Jun 2005; 272(11): 2901-2911.
  • Liew OW, et al. Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites. Protein Expr. Purif. Jun 2005; 41(2): 332-340.
  • Peng L, et al. High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris. J. Biotechnol. Mar 2004; 108(2): 185-192.
Full Name
Enterokinase, Light Chain, Porcine
Abbreviated Name-1Enterokinase
Abbreviated Name-2EK
DescriptionGenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.
SourceYeast
M.W.Theoretical MW: 21,880 Da
The apparent MW on SDS-PAGE: about 40,000 Da
Specific ActivityOne unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.
StorageStore at -20°C after delivery.
FormulationGenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.
Concentration50 ul, 2 U/ul
* For Non-Clinical Research Use Only *
1
 Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2
 Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3
 Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
4
 Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5
 Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.