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| Enterokinase, Light Chain, Porcine |
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| Catalog No. |
Size |
Price |
Figures |
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Z01003-100 IU
| 100 IU | $ 80.00 | MSDS: 20060502233525 (PDF)
PROTOCOL: 20061122042342 (PDF)
EXAMPLE:
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References:
- Palmai-Pallag T, et al. The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins. FEBS J. Jun 2005; 272(11): 2901-2911.
- Liew OW, et al. Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites. Protein Expr. Purif. Jun 2005; 41(2): 332-340.
- Peng L, et al. High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris. J. Biotechnol. Mar 2004; 108(2): 185-192.
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| Full Name | | Enterokinase, Light Chain, Porcine |
| | Abbreviated Name-1 | Enterokinase |
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| Abbreviated Name-2 | EK |
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| Description | GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by three aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags. |
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| Specific Activity | One unit is defined as the amount of enzyme needed to cleave 95% of 50 ug of fusion protein in 16 hours at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2. |
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| Storage | Store at -20°C after delivery. |
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| Formulation | GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity. |
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| Concentration | 50 ul, 2 U/ul |
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