| Catalog No. |
Size |
Price |
Figures |
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Z02021-10 ug
| 10 ug | $ 39.00 | COA: Z02021 (PDF)
References:
- Gessi M, et al. Parathyroid hormone-related peptide (hPTHrP) and parathyroid hormone-related peptide receptor type 1 (PTHR1) expression in human thymus. J. Histochem. Cytochem. Aug 2005; 53(8): 955-962.
- Huan J, et al. Parathyroid hormone 7-84 induces hypocalcemia and inhibits the parathyroid hormone 1-84 secretory response to hypocalcemia in rats with intact parathyroid glands. J. Am. Soc. Nephrol. Jul 2006; 17(7): 1923-1930.
- Mahon MJ, Segre GV. Stimulation by parathyroid hormone of a NHERF-1-assembled complex consisting of the parathyroid hormone I receptor, phospholipase Cbeta, and actin increases intracellular calcium in opossum kidney cells. J. Biol. Chem. May 2004; 279(22): 23550-23558.
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Z02021-50 ug
| 50 ug | $ 129.00 |
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Z02021-1 mg
| 1 mg | $ 800.00 |
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| Full Name | | Parathyroid Hormone (PTH) (1-84 PTH), human |
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| Abbreviated Name-1 | PTH |
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| Description | Parathyroid Hormone is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. Parathyroid Hormone is secreted from cells of the parathyroid glands and finds Parathyroid Hormone's major target cells in bone and kidney. Like most other protein hormones, parathyroid hormone is synthesized as a preprohormone. After intracellular processing, the mature hormone is packaged within the Golgi into secretory vesicles, the secreted into blood by exocytosis. Parathyroid hormone is secreted as a linear protein of 84 amino acids. Recombinant Human PTH produced in E. coli is a single, non-glycosylated, polypeptide chain containing 84 amino acids and having a molecular mass of 9,000 Da. |
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| M.W. | 9,000 Da |
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| Purity | Greater than 98.0% as determined by:
1. Analysis by RP-HPLC
2. Anion-exchange FPLC
3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
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| Endotoxin Level | Less than 0.1 ng/μg (IEU/μg) of PTH |
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| Specific Activity | PTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0×104 U/mg. |
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| Storage | Lyophilized PTH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
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| Formulation | The protein (1 mg/ml) was lyophilized after extensive dialyses against 1.15 mg sodium citrate, sodium chloride 7.31 mg, 0.21 mg citric acid, 0.1117 EDTA-Na2, 0.2 mg Tween 80 and 50 mg Mannitol. |
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| Reconstitution | It is recommended to reconstitute the lyophilized PTH in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
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