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| Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase |
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| Catalog No. |
Size |
Price |
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Z02032-10000 U
| 10000 U | $ 39.00 | MSDS: 20081030012707 (PDF)
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| Full Name | | Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase |
| | Abbreviated Name-1 | M-MLV RT |
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| Description | GenScript Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. The enzyme lacks 3’-5’ exonuclease activity. |
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| Source | E. coli |
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| Purity | Purity is > 90% as determined by SDS-PAGE. |
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| Reaction Buffer | 5X M-MLV RT Reaction Buffer: 250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2, and 50 mM DTT. |
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| Storage | Store the protein at -20°C. Avoid exposure to frequent temperature changes. |
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| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C.
The reaction conditions are as follows: 50 mM Tris-HCl (pH 8.3), 7 mM MgCl2, 40 mM KCl, 10 mM DTT, 0.1 mg/ml BSA, 0.5 mM [3H]dTTP, 0.025 mM oligo (dT), 0.25 mM poly(A), and 0.01% NP-40. |
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| Concentration | 200,000 units/ml in 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 1 mM DTT, 0.01% NP-40, 0.1 mM EDTA, 50% glycerol |
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| Quality Control | Activity testing shows no detectable DNase or RNase contamination. |
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