| Catalog No. |
Size |
Price |
Figures |
|---|
Z02036-10 ug
| 10 ug | $ 125.00 | MSDS: 20081030013034 (PDF)
|
|---|
Z02036-50 ug
| 50 ug | $ 390.00 |
|---|
Z02036-1 mg
| 1 mg | $ 2000.00 |
|---|
| Full Name | | Interleukin (IL)-10, mouse |
|
| Abbreviated Name-1 | rmIL-10 |
|---|
| Abbreviated Name-2 | IL-10 |
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| Description | Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL 10. In human IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by mococytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cells lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secerete large quantities of IL-10 into the conditioned medium. The synthesis of IL-10 by monocytes is inhibited by IL-4 and IL-10. GenScript Interleukin (IL)-10, mouse produced in E. coli is a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18,885 Da. |
|---|
| Source | E. coli |
|---|
| M.W. | 18,885 Da. |
|---|
| Purity | Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
|---|
| Endotoxin Level | Less than 0.1 ng/ug (IEU/ug) of mouse IL-10. |
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| Specific Activity | The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be <2 ng/ml, corresponding to a Specific Activity of 5×105 IU/mg. |
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| Storage | Lyophilized mouse IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine IL-10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1%HSA or BSA). Avoid freeze-thaw cycles. |
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| Formulation | The protein was lyophilized with no additives. |
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| Reconstitution | It is recommended to reconstitute the lyophilized mouse IL-10 in sterile 18 MΩ-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
|
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| Sequence analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln. |
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