| Catalog No. |
Size |
Price |
Figures |
|---|
Z02038-25000 U
| 25000 U | $ 130.00 | MSDS: 20081030013139 (PDF)
|
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| Full Name | |
| Abbreviated Name-1 | Hercules Endonuclease |
|---|
| Description | Hercules Endonuclease is a engineered endonuclease from Serratia marcescens. The endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2-5 bases in length. Hercules Endonuclease is compatible with Easy BacLysis Protein Extraction Solution. |
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| Source | E. coli |
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| Purity | Purity > 99% as determined by SDS-PAGE |
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| Storage | Stable at 4°C for several weeks, for long-term storage, store it at -20°C after receiving. No obvious decrease of enzyme activity can be found after an incubation at 37°C for a week. |
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| Unit Definition | One unit of Hercules Endonuclease is defined as the amount of enzyme that causes an A260 change of 1.0 in 30 minutes at 37°C, which corresponds to complete digestion of 37 μg of DNA. |
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| Concentration | 200 U/μl in 10 mM Tris-HCl, pH8.0, 2 mM MgCl2, 20 mM NaCl and 50% glycerol |
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| Applications | Viscosity reduction in protein extractsSample preparation for 2D gel electrophoresisRemoval of nucleic acid contaminants from recombinant protein preparations |
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