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Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RNase H-)

Catalog No. Size Price Figures
Z02040-10000 U
10000 U
$ 59.00
MSDS:  20081028224217  (PDF)

Full Name
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RNase H-)
Abbreviated Name-1M-MLV RT (RNase H-)
DescriptionGenScript Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RNase H-) is a genetically modified M-MLV Reverse Transcriptase (RT). It differs from the M-MLV Reverse Transcriptase (GenScript, Cat. No. Z02032) by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks ribonuclease H activity specific to RNA in RNA-DNA hybrids. RNase H activity is eliminated by a point mutation in the RNase H domain of M-MLV RT.
SourceE. coli
Purity>90%
Reaction Buffer5X M-MLV RT (RNase H-)Reaction Buffer: 250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2, and 50 mM DTT
StorageStore at -20°C. Avoid exposure to frequent temperature changes.
Unit DefinitionOne unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C.
The reaction conditions are: 50 mM Tris-HCl (pH 8.3), 7 mM MgCl2, 40 mM KCl, 10 mM DTT, 0.1 mg/ml BSA, 0.5 mM [3H]dTTP, 0.025 mM oligo (dT), 0.25 mM poly (A) and 0.01% NP-40.
Concentration200,000 units/ml in 20 mM Tris-HCl (pH7.5), 200 mM NaCl, 1 mM DTT, 0.01% NP-40, 0.1 mM EDTA, 50% Glycerol
Quality ControlNo detectable DNase and RNase contamination
* For Non-Clinical Research Use Only *
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