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| Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RNase H-) |
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| Catalog No. |
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Price |
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Z02040-10000 U
| 10000 U | $ 59.00 | MSDS: 20081028224217 (PDF)
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| Full Name | | Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RNase H-) |
| | Abbreviated Name-1 | M-MLV RT (RNase H-) |
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| Description | GenScript Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RNase H-) is a genetically modified M-MLV Reverse Transcriptase (RT). It differs from the M-MLV Reverse Transcriptase (GenScript, Cat. No. Z02032) by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks ribonuclease H activity specific to RNA in RNA-DNA hybrids. RNase H activity is eliminated by a point mutation in the RNase H domain of M-MLV RT. |
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| Source | E. coli |
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| Purity | >90% |
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| Reaction Buffer | 5X M-MLV RT (RNase H-)Reaction Buffer: 250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2, and 50 mM DTT |
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| Storage | Store at -20°C. Avoid exposure to frequent temperature changes. |
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| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C.
The reaction conditions are: 50 mM Tris-HCl (pH 8.3), 7 mM MgCl2, 40 mM KCl, 10 mM DTT, 0.1 mg/ml BSA, 0.5 mM [3H]dTTP, 0.025 mM oligo (dT), 0.25 mM poly (A) and 0.01% NP-40. |
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| Concentration | 200,000 units/ml in 20 mM Tris-HCl (pH7.5), 200 mM NaCl, 1 mM DTT, 0.01% NP-40, 0.1 mM EDTA, 50% Glycerol |
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| Quality Control | No detectable DNase and RNase contamination |
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