| Catalog No. |
Size |
Price |
Figures |
|---|
Z02041-2000 U
| 2000 U | $ 43.00 | |
|---|
| Full Name | |
| Abbreviated Name-1 | Ribonuclease Inhibitor |
|---|
| Description | Ribonuclease Inhibitor is a recombinant human placental protein which specifically inhibits ribonuclease (RNase) A, B and C. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA polymerase, AMV or M-MLV reverse transcriptase, or phage RNA polymerases (SP6, T7, or T3). |
|---|
| Source | E. coli |
|---|
| Purity | >95% |
|---|
| Storage | Store at -20°C. Avoid exposure to frequent temperature changes. |
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| Unit Definition | One unit is defined as the amount of RNase Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A. |
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| Concentration | 40,000 units/ml in 20 mM HEPES-KOH (pH 7.5), 50 mM KCl, 8 mM DTT and 50% glycerol. |
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| Quality Control | No detectable DNase, Endonuclease and RNase contamination. |
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| Applications | Applied in procedures where RNase contamination constitutes a problem:
* RT-PCR;
* cDNA synthesis;
* In vitro transcription/translation;
* RNA synthesis;
* Increase yield and activity of polysomes;
* Improvement of in vitro virus replication;
* Improvement of RNA translation in homologous systems;
* Preparation of RNase-free antibody. |
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