| Catalog No. |
Size |
Price |
Figures |
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Z02043-5 mg
| 5 mg | $ 150.00 | MSDS: 20081028055723 (PDF)
DATASHEET: 20090827025119 (PDF)
COA: Z02043_R313909 (PDF)
COA: Z02043_R60151010 (PDF)
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| Full Name | |
| Abbreviated Name-1 | Streptavidin |
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| Description | Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin can bind to four moles of biotin per mole of protein,and it shows extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does. |
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| Source | Streptomyces avidinii expressing in E. Coli
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| M.W. | 13,500 Da |
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| Isoelectric Point | pI of 6.52, 13.5 kD for each subunit (4 subunits in each molecule). |
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| Purity | This product is affinity purified by 2-IminoBiotin Resin (GenScrtipt，Cat. No. L00272) with the purity more than 95% and shows predominantly a single band by SDS-PAGE. |
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| Specific Activity | >15 U/mg protein, 1 U is defined as the quantity of protein that binds 1 microgramme |
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| Storage | The powder may be stored at 4°C for several weeks. For long-term storage, store at -20°C. |
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| Unit Definition | 1 unit is defined as the quantity of protein that can bind one microgram of biotin. |
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| Applications | Streptavidin can be used for the coating of solid phases (microtiter plates, beads, etc.) to immobilize or purify biotin-labeled or 2-iminobiotin-labeled molecules. Its conjugation with enzyme or fluorescein is widely used in immunological detection assays, such as the streptavidin/biotin system. |
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