| Catalog No. |
Size |
Price |
Figures |
|---|
Z02193-10 ug
| 10 ug | $ 130.00 | |
|---|
Z02193-50 ug
| 50 ug | $ 390.00 |
|---|
Z02193-1 mg
| 1 mg | $ 2340.00 |
|---|
| Full Name | | Interleukin (IL)-1α, mouse |
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| Abbreviated Name-1 | Interleukin (IL)-1α, mouse |
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| Description | IL-1α is distinct from the other agonist member of the IL-1 family, IL-1β. Although IL-1α triggers the same IL-1 receptor and although many of the biological effects of IL-1α are similar to those of IL-1α, in humans IL-1α is predominantly an intracellular molecule. In fact, there is evidence that IL-1α has both intracellular functions as a precursor molecule due to a nuclear localization sequence. IL-1α as an unprocessed precursor is biologically as active as the processed form. IL-1α is also found constitutively in epithelial cells, whereas constitutive expression of IL-1β is rare. In many ways, IL-1α appears to be closer to the fibroblast growth factor family than the secreted IL-1β form. Therapeutic strategies for blocking IL-1β predominate over those for blocking IL-1α. Many humans have circulating neutralizing antibodies to IL-1α but not IL-1β.
GenScript Interleukin (IL)-1α, mouse, produced in E. coli, is a non-glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of 18,000 Da. |
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| Source | E. coli |
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| M.W. | 18,000 Da |
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| Purity | The purity of GenScript Recombinant Mouse IL-1 Alpha is greater than 98% as determined by the following methods:
(a) RP-HPLC analysis
(b) Anion-exchange FPLC
(c) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
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| Endotoxin Level | The endotoxin level of GenScript Recombinant Mouse IL-1 Alpha is below 0.1 ng/µg (1 IEU/µg) of IL-1α. |
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| Storage | Although lyophilized GenScript Recombinant Mouse IL-1 Alpha can remain stable at room temperature for up to three weeks, it is best stored desiccated below -18°C. After reconstitution, GenScript Recombinant Mouse IL-1 Alpha may be stored at 4°C for up to seven days and below -18°C for longer periods. For long-term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added. Please avoid freeze-thaw cycles. |
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| Formulation | GenScript Mouse IL-1α is lyophilized after extensive dialysis against PBS. |
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| Reconstitution | It is recommended that the lyophilized GenScript Recombinant Mouse IL-1 Alpha be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
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| Specificity | The ED50, as determined by the dose-dependant stimulation of mouse D10S cells, was found to be less than 0.01 ng/ml, corresponding to a specific activity of 1.0×108 IU/mg. |
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| Sequence | The sequence of the first five N-terminal amino acids has been found to be Ser-Ala-Pro-Tyr-Thr. |
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