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5' cohesive DNA termini the single-stranded, complementary regions of DNA that are formed when both of the strands of a double-stranded DNA molecule are cleaved in an asymmetric manner. The Restriction endonuclease EcoRI, for example, cleaves the sequence "GAATTC" between the G and A to generate the 4-base, 5'-cohesive terminus "AATT". EcoRI.GIF

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